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Recombinant Proteins> | … Cell transfection. Cells were all cultured in the medium for 24 h and seeded into 96-well plate for transfection. miR-146b mimic (20 nmol/L),miR-146b inhibitor (20 nmol/L) and negative control were all obtained from Genscript and were used according to the protocol … | Get A Quote |
Prostate cancer (PCa) is considered as a common visceral cancer in males and the sixth major cause of cancer-related deaths in males worldwide. Significant diagnostic and therapeutic advances have been made in the past decades. However, an improved understanding of their molecular mechanism is still needed. In the present research, we first detected the expression of miR-146b by quantitative real-time PCR (qRT-PCR) and found that miR-146b expression was increased in PCa. Subsequently, we found that miR-146b play an important role in the viability and proliferation capacity of PCa cells functionally. To explore the mechanism, we performed western blot to examine the autophagy-related markers, and found... More